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Antioxidant and Antiplatelet Effects of Dang-Gui-Shao-Yao-San on Human Blood Cells

Ai-Yu Shen

Basic Medical Science Education Center, Fooyin University, Ta-Liao, Kaohsiung County, Taiwan, ROC

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Trey-Shy Wang

Basic Medical Science Education Center, Fooyin University, Ta-Liao, Kaohsiung County, Taiwan, ROC

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Mei-Han Huang

Basic Medical Science Education Center, Fooyin University, Ta-Liao, Kaohsiung County, Taiwan, ROC

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Chang-Hui Liao

Graduate Institute of Natural Products, Chang Gung University, Tao-Yuan 333, Taiwan, ROC

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Sheue-Jiun Chen

Basic Medical Science Education Center, Fooyin University, Ta-Liao, Kaohsiung County, Taiwan, ROC

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, and

Chun-Ching Lin

Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC

Correspondence to: Dr. Chun-Ching Lin, Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. Tel: (+886) 7-312-1101 (ext. 2122).

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https://doi.org/10.1142/S0192415X05003351Cited by:30 (Source: Crossref)

Abstract

Dang-Gui-Shao-Yao-San (DGSYS) is a mixture of medicinal herbs, which has long been used in traditional Chinese medicine for treating anemia and ovulary disorders. Its preparation comprises Angelicae sinensis (Oliv.) Diels, Ligustucum chuanxiong Hort, Paeonia lactiflora pall, Poria cocos (Schw.) Wolf, Atractylodis macrocephala Koidz and Alisma orientalis (Sam.) Juzep. The present study examined the anti-superoxide formation, free radical scavenging and anti-lipid peroxidation activities of DGSYS by xanthine oxidase inhibition, cytochrome C system with superoxide anion released by the fMLP or PMA activating pathway in human neutrophils, and FeCl₂ ascorbic acid-induced lipid peroxidation effects on lipids in rat liver homogenate, respectively. DGSYS showed anti-superoxide formation and free radical scavenging activity in a concentration-dependent manner. It also inhibited PMA- but not fMLP-induced superoxide anion released from human neutrophils. These antioxidant actions of DGSYS showed beneficial cytoprotective effects against lipid peroxidation in rat liver homogenate, human platelet aggregation induced by arachidonic acid (AA) and adenosine diphosphate (ADP) and mitomycin C-mediated hemolytic in human erythrocytes.

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