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Curcumin and Saikosaponin A Inhibit Chemical-Induced Liver Inflammation and Fibrosis in Rats

Shu-Ju Wu

School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan

Department of Nursing, Chang Gung Institute of Technology, Taoyuan 333, Taiwan

The first two authors contributed equally to this work.

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Ka-Wai Tam

Division of General Surgery, Department of Surgery, Taipei 110, Taiwan

The first two authors contributed equally to this work.

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Ya-Hui Tsai

School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan

School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan

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Chun-Chao Chang

Division of Gastroenterology, Taipei 110, Taiwan

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, and

Jane C.-J. Chao

School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan

Nutrition Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan

Correspondence to: Dr. Jane C.-J. Chao, School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. Tel: (+886) 2-2736-1661 (ext. 6548), Fax: (+886) 2-2737-3112.

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https://doi.org/10.1142/S0192415X10007695Cited by:90 (Source: Crossref)

Abstract

Curcumin and saikosaponin A as antioxidants improve antioxidant status. This study investigated the anti-inflammatory and antifibrotic actions of curcumin and saikosaponin A on CCl₄-induced liver damage. Sprague-Dawley rats were randomly divided into control, CCl₄, CCl₄+ curcumin (0.005%; CU), CCl₄ + saikosaponin A (0.004%; SS), and CCl₄ + curcumin + saikosaponin A (0.005% + 0.004%; CU + SS) groups. Carbon tetrachloride (40% in olive oil) at a dose of 0.75 ml/kg was injected intraperitoneally once a week. Curcumin and saikosaponin A were supplemented alone or in combination with diet 1 week before CCl₄ injection for 8 weeks. After 8-week supplementation, histopathological results showed hepatic collagen deposition was significantly reduced in the CU and SS groups, and activated nuclear factor-κ B expression induced by CCl₄ in the liver was significantly inhibited by curcumin and/or saikosaponin A. Hepatic proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 were significantly inhibited, and anti-inflammatory cytokine interleukin-10 was significantly increased by supplementation with curcumin and/or saikosaponin A. Additionally, curcumin and/or saikosaponin A significantly reduced the increased levels of hepatic transforming growth factor-β1 and hydroxyproline after CCl₄ treatment. Therefore, supplementation with curcumin and/or saikosaponin A suppress inflammation and fibrogenesis in rats with CCl₄-induced liver injury. However, the combination has no additive effects on anti-inflammation and antifibrosis.

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