Curiosity and Passion for Science and Art

The following sections are included:

• FOREWORD
• CONTENTS

The following sections are included:

• My Interests During My Formative Years
• Freeze-Etching Methods
• Early S-Layer Work
• S-Layer Glycobiology
• S-Layers as Molecular Sieves
• Composite S-Layer Lipid Structures
• S-Layers as Patterning Structure for Various Uses
• A Few Final Remarks Concerning the History of S-Layer Research

My scientific activities have never been my sole focus of interest. Looking back, my inspiration for artistic activities started quite early in my life. The walls in our home were full of oil paintings and watercolor pictures, mostly by my father, but some were from other artists, which my father had obtained in exchange for his own work. I do not know if it was the effect of such an environment or if I had inherited the proper genes (epigenetics), but already in elementary school my teachers noticed that I was very gifted in making fanciful drawings of animals and people. My father not only introduced me to different techniques for painting and drawing, but also explained to me how to plan compositions. I was quite motivated to try different techniques and to improve my performance. Moreover, while drawing or painting I could retreat into a subconscious world of fertile imagination, or “flow,” using the prevailing term of psychology…

The following are references for all original, peer-reviewed papers, invited reviews, and book chapters (status at January 31, 2016), ordered by year. Those reproduced in this book are marked by underlining the name of the first author. The list also includes some references to the work of other groups, provided for context (marked in italics)…

REPRINTS OF SELECTED ARTICLES IN CHRONOLOGICAL ORDER as follows:

• 1. Sleytr, U.B. and A.M. Glauert (1973) Evidence for an empty core in a bacterial flagellum. Nature 241: 542–543.
• 2. Sleytr, U.B. and A.M. Glauert (1975) Analysis of regular arrays of subunits on bacterial surfaces; evidence for a dynamic process of assembly. J. Ultrastruct. Res. 50: 103–116.
• 3. Sleytr, U.B. (1975) Heterologous reattachment of regular arrays of glycoproteins on bacterial surfaces. Nature 257: 400–402.
• 4. Sleytr, U.B. and K.J.I. Thorne (1976) Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum. J. Bacteriol. 126: 377–383.
• 5. Sleytr, U.B. and A.M. Glauert (1976) Ultrastructure of the cell walls of two closely related Clostridia that possess different regular arrays of surface subunits. J. Bacteriol. 126: 869–882.
• 6. Crowther, R.A. and U.B. Sleytr (1977) An analysis of the fine structure of the surface layers from two strains of Clostridia, including correction for distorted images. J. Ultrastruct. Res. 58: 41–49.
• 7. Sleytr, U.B. and A.W. Robards (1977) Plastic deformation during freeze-cleavage: a review. J. Microsc. 110: 1–25.
• 8. Küpcü, Z., L. März, P. Messner and U.B. Sleytr (1984) Evidence for the glycoprotein nature of the crystalline cell wall surface layer of Bacillus stearothermophilus strain NRS 2004/3a. FEBS Lett. 173: 185–190.
• 9. Messner, P., D. Pum, M. Sára, K.O. Stetter and U.B. Sleytr (1986) Ultrastructure of the cell envelope of the archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus. J. Bacteriol. 166: 1046–1054.
• 10. Christian, R., G. Schulz, F.M. Unger, P. Messner, Z. Küpcü and U.B. Sleytr (1986) Structure of a rhamnan from the surface-layer glycoprotein of Bacillus stearothermophilus strain NRS 2004/3a. Carbohydr. Res. 150: 265–272.
• 11. Sleytr, U.B. and M. Sára (1986) Ultrafiltration membranes with uniform pores from crystalline bacterial cell envelope layers. Appl. Microbiol. Biotechnol. 25: 83–90.
• 12. Messner, P., D. Pum and U.B. Sleytr (1986) Characterization of the ultrastructure and the self-assembly of the surface layer of Bacillus stearothermophilus strain NRS 2004/3a. J. Ultrastruct. Mol. Struct. Res.97: 73–88.
• 13. Sára, M. and U.B. Sleytr (1987) Molecular sieving through S-layers of Bacillus stearothermophilus strains. J. Bacteriol. 169: 4092–4098.
• 14. Sára, M. and U.B. Sleytr (1988) Membrane biotechnology: two-dimensional protein crystals for ultrafiltration purposes. In: H.-J. Rehm (ed.), Biotechnology, 6b, VCH, Weinheim, pp. 615–636.
• 15. Sára, M., S. Küpcü and U.B. Sleytr (1989) Localization of the carbohydrate residue of the S-layer glycoprotein from Clostridium thermohydrosulfuricum L111-69. Arch. Microbiol. 151: 416–420.
• 16. Pum, D., P. Messner and U.B. Sleytr (1991) Role of the S-layer in morphogenesis and cell division of the archaebacterium Methanocorpusculum sinense. J. Bacteriol. 173: 6865–6873.
• 17. Küpcü, S., M. Sára and U.B. Sleytr (1993) Influence of covalent attachment of low molecular weight substances on the rejection and adsorption properties of crystalline proteinaceous ultrafiltration membranes. Desalination 90: 65–76.
• 18. Ries, W., C. Hotzy, I. Schocher, U.B. Sleytr and M. Sára (1997) Evidence that the N-terminal part of the S-layer protein from Bacillus stearothermophilus PV72/p2 recognizes a secondary cell wall polymer. J. Bacteriol.179: 3892–3898.
• 19. Shenton, W., D. Pum, U.B. Sleytr and S. Mann (1997) Synthesis of cadmium sulphide superlattices using self-assembled bacterial S-layers. Nature 389: 585–587.
• 20. Sára, M., C. Dekitsch, H.F. Mayer, E.M. Egelseer and U.B. Sleytr (1998) Influence of the secondary cell wall polymer on the reassembly, recrystallization and stability properties of the S-layer protein from Bacillus stearothermophilus PV72/p2. J. Bacteriol. 180: 4146–4153.
• 21. Moll, D., C. Huber, B. Schlegel, D. Pum, U.B. Sleytr and M. Sára (2002) S-layer-streptavidin fusion proteins as template for nanopatterned molecular arrays. Proc. Natl. Acad. Sci. U.S.A. 99: 14646–14651.
• 22. Pum, D. and U.B. Sleytr (2009) S-layer proteins for assembling ordered nanoparticle arrays. In: A. Offenhäusser and R. Rinaldi (eds.). In the series Nanostructure Science and Technology. D. Lockwood (ed.), Nanobioelectronics for Electronics, Biology, and Medicine. Springer, Berlin, pp. 167–180.
• 23. Schuster, B. and U.B. Sleytr (2009) Composite S-layer lipid structures. J. Struct. Biol. 168: 207–216.
• 24. Messner, P., E.M. Egelseer, U.B. Sleytr and C. Schäffer (2009) Bacterial surface layer glycoproteins and “non-classical” secondary cell wall polymers. In: A.P. Moran, P.J. Brennan, O. Holst and M. von Itzstein (eds.), Microbial Glycobiology: Structure, Relevance and Application. Academic– Elsevier, San Diego, pp. 109–128.
• 25. Ilk, N., E.M. Egelseer and U.B. Sleytr (2011) S-layer fusion proteins — construction principles and applications. Curr. Opin. Biotech. 22(6): 824–831.
• 26. Schuster, B. and U.B. Sleytr (2014) Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules. J. R. Soc. Interface. 11: 20140232.
• 27. Sleytr, U.B., B. Schuster, E.-M. Egelseer and D. Pum (2014) S-layers: principles and applications. FEMS Microbiol. Rev. 38: 823–864. DOI: 10.1111/1574-6976.12063.
• 28. Pum, D. and U.B. Sleytr (2014) Reassembly of S-layer proteins. Nanotechnology 25: 312001. DOI: 10.1088/0957-4484/25/31/312001.
• 29. Schuster, B. and U.B. Sleytr (2015) Relevance of glycosylation of S-layer proteins for cell surface properties. Acta Biomater. 19: 149–157. DOI: 10.1016/j.actbio.2015.03.020.

SELECTED ARTWORK on:

• SCULPTURES GILDED WITH LEAF GOLD
• SPLASHED SCULPTURES
• SCULPTURES DISTORTED IN MIRROR FOIL
• SCULPTURES WITH ABSTRACT OVERLAY
• IMAGES DISTORTED IN MIRROR FOILS

The following sections is included:

• Corpus Acknowledgments