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Preparation of chlorin e6–monoclonal antibody conjugate and its effect for photodynamic therapy

SHUN-ICHIRO OGURA

Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

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Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

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TOSHIAKI KAMACHI

Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

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Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

Corresponding author, Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.

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https://doi.org/10.1002/jpp.351Cited by:12 (Source: Crossref)

Abstract

Chlorin e6 (Ce6) was conjugated with anti-tumor monoclonal antibody (IgG2b) to increase its binding affinity for tumors. The Ce6 was activated by N-hydroxysuccinimide and directly conjugated with IgG2b (IgG2b–Ce6) via peptide bonds. An average of 11 Ce6 molecules were coupled to each IgG2b molecule. IgG2b–Ce6 showed high cellular uptake in tumor cells and high photocytotoxicity. These results indicate IgG2b–Ce6 is a good photosensitizer with high binding affinity to tumor cells.

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Journal cover image

Vol. 05, No. 05

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History

Received 28 August 2000

Revised 7 November 2000

Accepted 18 November 2000

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