Fluorescent Probes

The following sections are included:

• ION PROBES

  • Calcium Probing

  • Calcium Compartmentalization in the Sarcoplasmic and Endoplasmic Reticulum: Spatially and Functionally Distinct Calcium Stores

  • Localization of Sodium/Calcium Exchanger in rat cortical astrocytes

  • pH Probes

    • Videomicrofluorometry of pH in Single Living Cells

    • Yolk Platelet pH Studies During Xenopus laevis Development

• MEMBRANE POTENTIAL PROBES

  • Confocal Microscopy of Potentiometric Dyes

• CELL ORGANELLE PROBES

  • Mitochondrial Fluorescence Probes

    • Quantitative Differential Effects of Mitochondrial Probe Rhodamine 123 on Human Normal Cells and Colon Cancer Cells

      • Aberrant Mitochondria in Colon Carcinoma Lines

    • Monitoring of Relative Mitochondrial Membrane Potential in Living Cells by Fluorescence Microscopy and Perturbation of Rhodamine 123 Accumulation in Mitochondria

    • Rhodamine 123 and Analogs on Function and Viability of Pulsating Myocardial Cells

    • Resistance to Rhodamine 123

    • Mitochondrial Alterations in Cancerous Cells and Targets for Therapy

    • Localization of Mitochondria with Rhodamine 123 and Its Significance in Oncology

    • A New Mitochondrial Probe: J-Aggregate Formation of the Lipophilic Cation JC-1

    • Simultaneous Imaging of Cell and Mitochondrial Membrane Potentials

    • Membrane Potentials in Individual Mitochondria with Imaging in Five Dimensions

    • Cytosolic Calcium Transients and Concurrent Mitochondrial Depolarizations

  • Golgi Apparatus

    • The Endoplasmic Reticulum-Golgi Membrane System ER-Golgi Trafficking

    • Golgi Studies in Hepatocytes and Fibroblasts

    • Golgi Studies in Fusion-Competent and Fusion-Deficient Myoblasts

  • Endosomes

    • Transbilayer Movement of BODIPY-labeled Lipids in Lipid Vesicles

    • A Modulator of Endocytosis: Ceramide

    • Non-Endocytic Uptake of Fluorescent Analogs of Phosphatidylserine

    • The Acidification of the Cytosol-Stimulated C6-NDP-PS Uptake in Chinese Hamster (CHO) Fibroblasts

    • Spectral Properties of Plasma Membrane Lipid Analog; the First Seconds of Endocytosis

    • Transferrin Endocytic Sorting

    • Retardation of Transferrin Receptor Recycling by Bafilomycin A

  • Lysosomal Probes

    • Cell-Permeant Probes for Lysosomes

    • Other Lysosomotropic Probes

    • Fluorogenic Probes of Lysosomal Hydrolases

    • Fluorescent Probes of Lysosomal Hydrolases

    • Lysosomes in Cells Treated with Cytotoxic Agents

  • Endoplasmic Reticulum (ER)

    • Construction of the Endoplasmic Reticulum

    • Dynamic Behavior of ER in Living Cells

    • Dynamics of the ER in Nonmuscle and Muscle Cells

    • Dynamics of the Cytoskeleton-Organelle Connections

  • Probes of Cytoskeleton

    • Probes for Microtubules

      • Microinjection of Probes for Microtubules

      • Microtubule Stress Fibers and Endoplasmic Reticulum Interaction

      • Effects of Microtubules on Mitochondrial Distribution

      • Distribution of Microtubules and Inactivation of Organelle Motility in Cells Photosensitized by 3-3'-dihexyl Carbocyanine Iodide [DiOC₆(3)]

      • Midzone Microtubule Bundles and Cytokinesis

    • Microfilaments (actin)

      • Actin Assembly in the Cortical Region of Growing Neurites in PC-12 Cells

      • Dynamics of Nonfilamentous Actin

      • Cortical Movement of Microinjected Actin in the Formation of Contractile Ring in Dividing Cultured Animal Cells

      • Actin and α-Actinin Along Stress Fibers

      • Possible Role of Treadmilling in Exchange of Actin Subunits at the Leading Edge of Living Fibroblasts

      • Exchangeability of α-Actinin in Cardiac Fibroblasts and Myocytes

      • α-Actinin Aggregates in Transformed Cells

      • Cytokinesis and 3-D Orientation of Actin Filaments

      • Surface Receptor Movement During Cell Division

      • Spindle Dynamics and Cytokinesis in Vitro

      • Localization of Profilin in Living Cells

    • Myosin

      • Formation of Myosin-Containing Filaments in 3T3 Fibroblasts

      • Studies with Labeled Myosin in Myotubes

      • Premyofibrils in Cultured Adult Cardiomyocytes

      • Force Transmission at the Site of Costameres in Rat Cardiomyocytes

      • Myofibrillogenesis in Living Embryonic Cardiomyocytes Transfected with Plasmids Expressing Green Fluorescent Protein (GFP) Linked to α-Actinin

      • Talin Dynamics in Nonmuscle Cells

      • Localization of the Fusion Construct (Titin-Fragment-GFP) in the Z-Bands of Myocytes: Overexpression and Disassembly

      • Zeugmatin in the Z-band Targeting Region of Titin

    • Probes of the Intermediate Filaments

      • Cytoskeletal-Extracellular Matrix Interactions

      • Disruption of Microfilament Organization by Microinjection of Plasma Vitamin D-Binding Protein

    • Fluorescent Probes of Cell Division

    • Cell Motility in Living Cells

  • Probes of Membrane Anchoring

• PROBES OF CELL METABOLISM IN THE LIVING CELL, AS A RECEPTACLE OF SMART PROBES

• PROBES OF CYCLIC AMP (cAMP) PROBING, SIGNALING AND OSCILLATIONS

• NUCLEIC ACID PROBES

  • Fluorescent Oligonucleotide Conjugates

  • Cyanine Fluorophores

  • Live Cell Nucleic Acid Stains

  • Molecular Mechanisms Controlling the Incorporation of Fluorescent Nucleotides Into DNA by PCR

• PROBES OF OXYGENATION AND ANOXIA

• FLUORESCENT PROBES OF REDUCED GLUTATHIONE AND THIOL GROUPS

  • New Thiol Reagents

• MEROCYANINES

• CAGED SUBSTRATES AND PHOTOACTIVATED PROBES

  • Intracellular Calcium Signaling Studied by Caged Compounds

  • Calcium Waves Synchronized by Mitochondrial Substrates

• OPTICAL THERMOMETRY IN SINGLE LIVING CELLS

  • NBD-liposomes

• REFERENCES