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INFLUENCE OF MELATONIN ON HaCaT CELL PROLIFERATION MEASURED BY MEANS OF ATP BIOLUMINESCENCE AND FLUOROMETRIC ASSAYS

    https://doi.org/10.1142/9789812776624_0109Cited by:0 (Source: Crossref)
    Abstract:

    The epiphysial hormone melatonin is characterized by many pharmacological effects in biological systems. In vitro-investigations to characterize the growth of spontaneously immortal human HaCaT-keratinocytes (Deutsches Krebsforschungszentrum, Heidelberg, Germany) influenced by melatonin were performed by Nickel et al.1 Evaluation of the cytotoxic effects after application of different substances was assessed by the DNA-synthesis rate which was determined by BrdU – insertion. The decrease of the DNA synthesis rate could be shown after treatment of keratinocytes with melatonin in high concentrations (1 nM). However, low concentrations of melatonin ( 10 μM -1 nM ) caused an increase of BrdU – insertion after 24h. The research laboratory of the Department of Dermatology (Friedrich-Schiller-University of Jena) found the inhibitory as well as the stimulating effects of melatonin depend on its concentration for growth of hair. A stimulation of hair growth could be detected in concentrations between 10 and 40 μmol in comparison to William's E medium. The maximum of the stimulating effect was found at 30 μmol.2 The aim of the present study was to determine the optimal melatonin doses for increasing HaCaT cell proliferation…