ENZYMES IN PREPARING CYCLODEXTRINS

The following sections are included:

• Introduction

• CGTase

  • The catalytic mechanism of CGTase

  • Classification of CGTase and their bacteria sources

  • The enzymatic properties of CGTase

• Preparation of CGTase by Fermentation

  • The types and the expansion of cultivation of the bacteria for preparation of CGTase

  • Control of fermentation conditions of CGTase

  • Determination of CGTase activity

• Purification of CGTase

  • Pretreatment and filtration of fermentation broth

  • Concentration and precipitation of crude enzyme solution

  • Enzyme purification

• The Substrate Catalysis Characteristics and Sources of Pullulanase

  • The substrate catalysis characteristics pullulanase

  • Source of pullulanase

• The Substrate Catalysis Characteristics and Sources of Isoamylase

  • The substrate catalysis characteristics and of isoamylase

  • The source of isoamylase

• The Source and Nature of Galactosidase

  • α-Galactosidase

  • α-galactosidase

• Immobilization of the Enzymes for CD Preparation

  • Preparation of immobilized enzyme

  • Properties and application of immobilized enzyme

• References