METHOD FOR DETERMINING THE LOCALIZATION OF TRACE ELEMENTS OBSERVED BY THE MICRO PIXE CAMERA
Abstract
The method to observe the localization of the trace elements in cultured human cell, by the micro Particle Induced X-ray Emission (PIXE) camera, was developed.
The analyzed samples received the eosin staining, which colored the nucleus of cell violet. The scanned areas of the sample with particle irradiation were colored brown by the heat during irradiation. The brown scanned areas were examined by an optical microscope at 400 X magnification and photographed. Then the photo was digitized and loaded into the microcomputer. By microcomputer, the photo of the scanned area was superimposed onto the image of the micro PIXE camera, by matching the scanned area to the image of micro PIXE camera.
This superimposed image enabled us to determine whether the elements exists in cytosol, or in nucleus.
