The present study was carried out with the aim to determine if, orthodontic patients accumulate measurable concentration of Ni in blood or not, since the recent evidences shows the allergenic actions of Ni in various forms and orthodontic appliances have been reported to produce Ni allergy. In our experiment, the blood samples were taken before the insertion of appliance and at an interval of 6 months over a total time period of 18 months (four sets) from the Oral Health Department of Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India. In all the four sets of blood samples the common trace elements were detected viz. S, K, Ca, Cr, Fe, Cu, Zn and Br. Our result shows the complete absence of Ni in the blood.

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