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3D imaging of human cells using $P I X E μ C T$ $P I X E μ C T$

S. Matsuyama

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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K. Ishii

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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S. Toyama

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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K. Watanabe

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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S. Koshio

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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K. Kasahara

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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S. Ito

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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A. Terakawa

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

E-mail Address: atsuki.terakawa@qse.tohoku.ac.jp

Corresponding author.

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M. Fujiwara

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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Y. Suzuki

Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Miyagi 980-8579, Japan

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A. Carmona

CENBG, CNRS University of Bordeaux, BP120 Le Haut Vigneau, 33175 Gradignan, France

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S. Roudeau

CENBG, CNRS University of Bordeaux, BP120 Le Haut Vigneau, 33175 Gradignan, France

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, and

R. Ortega

CENBG, CNRS University of Bordeaux, BP120 Le Haut Vigneau, 33175 Gradignan, France

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https://doi.org/10.1142/S0129083514500089Cited by:3 (Source: Crossref)

Abstract

We report imaging of human lung epithelial cells exposed to cobalt oxide ( ${C o}_{3} O_{4}$ ) microparticles using three-dimensional (3D) particle-induced X-ray emission microcomputed tomography ( $P I X E μ C T$ ). The use of energy-selectable quasi-monochromatic low-energy X-rays generated via proton microbeam bombardment led to high-quality images. We also carried out two-dimensional (2D) micro-PIXE imaging. The 3D $P I X E μ C T$ imaging data are complementary with 2D micro-PIXE images and the CT value ratios of the cells show that the strong absorption stems from ${C o}_{3} O_{4}$ . The 2D micro-PIXE analysis provides projection images of the elemental distribution, and this in combination with the 3D $P I X E μ C T$ imaging revealed details of the internal distribution of ${C o}_{3} O_{4}$ , and hence provides insight into the mechanisms of ${C o}_{3} O_{4}$ toxicity via intracellular perinuclear accumulation.