LIGHT EMITTING DIODES IN BIO-IMAGING
Abstract
Light-emitting diodes (LEDs) can and are currently integrated into light microscopes. They have numerous advantages as illumination sources. Most notably, they provide intensity (brightness) and spectral control during bio-imaging. For transmitted light imaging, LEDs can replace the traditional tungsten filament bulb, while offering longer life, little-to-no color temperature shift resulting from an intensity change, reduced emission in the infrared region, (a property important for live cell imaging), and reduced cost of ownership. For fluorescent imaging, in which the typical illumination sources are mercury or xenon lamps, LEDs offer the advantages of a longer lifespan, greater spatial and temporal stability, elimination of the need for mechanical shutters and neutral density filters, significantly lower cost of ownership, and reduction of photon dose at the specimen. Additionally, LEDs permit vibration-free, high-speed spectral and temporal modulation. This modulation allows more information to be obtained for a given photon dose.
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