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Effect of Dang-Gui-Ji-Hwang-Yeum on Osteoporosis in Ovariectomized Rats

Department of Pharmacology and the Institute of Cardiovascular Research, School of Medicine, Chonbuk National University, Jeonju, Chonbuk 565-701, South Korea

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Dong-Hyeon Yun

Department of Dental Pharmacology and Wonkwang Dental Research Institute, School of Dentistry, Wonkwang University, Iksan, Chonbuk 570-749, South Korea

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Hee-Young Chin

Department of Dental Pharmacology and Wonkwang Dental Research Institute, School of Dentistry, Wonkwang University, Iksan, Chonbuk 570-749, South Korea

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Sim-Keum Yoo

Department of Gynecology, School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk 570-749, South Korea

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Hyung-Min Kim

Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, South Korea

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Song-Baeg Kim

Department of Gynecology, School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk 570-749, South Korea

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, and

Hyung-Ryong Kim

Department of Dental Pharmacology and Wonkwang Dental Research Institute, School of Dentistry, Wonkwang University, Iksan, Chonbuk 570-749, South Korea

Correspondence to: Dr. Hyung-Ryong Kim, Chairman and Department of Dental Pharmacology, Wonkwang University, School of Dentistry, Iksan, Chonbuk 570-749, South Korea. Tel: (+82) 63-850-6640, Fax: (+82) 63-854-0285.

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https://doi.org/10.1142/S0192415X04002004Cited by:3 (Source: Crossref)

Abstract

Dang-Gui-Ji-Hwang Yeum (DGJHY; an Oriental prescription), has been successfully used for the management of osteoporotic disorders in China, Japan and Korea. In this study, we have characterized the effect of DGJHY on osteoporosis-associated phenomena in ovariectomized (OVX) rats by measuring body weights and bone histomorphometries in sham, OVX and DGJHY-administered OVX rats. Light microscopic analyses showed a porous or an eroded appearance on the tibial trabecular bone surface in OVX rats, whereas those of the sham and DGJHY-administered OVX rats were composed of fine particles. The trabecular bone area and the trabecular thickness in OVX rats were significantly lower than those of sham rats. Moreover, these reductions in OVX rats were significantly reversed by the administration of DGJHY for 7 weeks, and osteoclast numbers were also significantly reduced. Although no differences were observed between OVX and DGJHY-administered OVX rats and the sham animals at the T₃ level, we have found significant differences between these two groups at the T₄ level. However, serum phosphorus, calcium, mechanical strength and the surface appearance of osteoblasts in the DGJHY-administered OVX rats were similar to those of OVX rats. These results suggest that DGJHY is effective at preventing bone loss in OVX rats.

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References

E. Braunwaldet al., Harrison's Principles of Internal Medicine, 15th edn. 2 (McGraw-Hill Publishing Co., USA, 2001) pp. 2060–2066. Google Scholar
C. H. Chesnut 3et al., Metabolism 32, 571 (1983), DOI: 10.1016/0026-0495(83)90027-6. Crossref, Medline, Web of Science, Google Scholar
D. W. Dempsteret al., Bone 16, 157 (1995), DOI: 10.1016/S8756-3282(94)00024-7. Crossref, Medline, Web of Science, Google Scholar
B. Ettinger, H. K. Genant and C. E. Cann, Ann. Intern. Med. 106, 40 (1987). Crossref, Medline, Web of Science, Google Scholar
P. Fantiet al., Osteoporos. Int. 8, 274 (1988), DOI: 10.1007/s001980050065. Crossref, Medline, Web of Science, Google Scholar
R. P. Heaney and P. D. Saville, Clin. Pharmacol. Ther. 20, 593 (1976). Crossref, Medline, Web of Science, Google Scholar
F. Ismailet al., Endocrinology 122, 2624 (1989). Google Scholar
R. R. Liu, Harden and B. W. Hollis, Endocrinology 124, 7 (1989). Medline, Web of Science, Google Scholar
D. N. Kaluet al., Bone Miner. 14, 175 (1991), DOI: 10.1016/0169-6009(91)90021-Q. Crossref, Medline, Google Scholar
D. N. Kalu, Bone Miner. 15, 175 (1991), DOI: 10.1016/0169-6009(91)90124-I. Crossref, Medline, Google Scholar
D. B. Kimmel and T. J. Wronski, Calcif. Tissue Int. 45, 101 (1990). Google Scholar
N. Koyamaet al., J. Immunol. Methods 139(1), 17 (1991), DOI: 10.1016/0022-1759(91)90346-H. Crossref, Medline, Web of Science, Google Scholar
A. W. Kung and S. S. Yeung, J. Clin. Endocrinol. Metab. 81(3), 1232 (1996), DOI: 10.1210/jc.81.3.1232. Medline, Web of Science, Google Scholar
G. F. Mazzuoliet al., Calcif. Tissue Int. 38, 3 (1986), DOI: 10.1007/BF02556587. Crossref, Medline, Web of Science, Google Scholar
L. Mosekildeet al., Acta Endocrinol. (Copenh.) 85, 515 (1977). Crossref, Medline, Google Scholar
L. Mosekilde, E. F. Eriksen and P. Charles, Endocrinol. Metab. Clin. North Am. 19, 35 (1990). Crossref, Medline, Web of Science, Google Scholar
K. Terasawa, H. Kondo and N. Nagamachi, Nippon Sanka Fujinka Gakkai Zasshi 45, 1155 (1993). Medline, Google Scholar
L. Wartofsky, Thyroid Today 11, 1 (1988). Web of Science, Google Scholar
T. N. Wronski, M. Cintron and L. M. Dann, Calcif. Tissue Int. 43, 179 (1988), DOI: 10.1007/BF02571317. Crossref, Medline, Web of Science, Google Scholar
T. J. Wronskiet al., Bone Miner. 18, 133 (1992). Medline, Google Scholar
R. J. Xiu, JAMA 260, 1755 (1988), DOI: 10.1001/jama.260.12.1755. Crossref, Medline, Web of Science, Google Scholar