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"Tien-Hsien Liquid" Can Modulate Antigen-Stimulated Cytokine Production by T-Cells Isolated from Patients with Recurrent Aphthous Ulcerations

Andy Sun

School of Dentistry, National Taiwan University, Taipei, Taiwan, ROC

Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan, ROC

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Jean-San Chia

School of Dentistry, National Taiwan University, Taipei, Taiwan, ROC

Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC

Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan, ROC

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Won-Bo Wang

Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC

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, and

Chun-Pin Chiang

School of Dentistry, National Taiwan University, Taipei, Taiwan, ROC

Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan, ROC

Correspondence to: Dr. Chun-Pin Chiang, School of Dentistry, National Taiwan University Hospital, No. 1, Chang-Te Street, Taipei, Taiwan. Tel: (+886) 2-2312-3456 (ext. 6855), Fax: (+886) 2-2389-3853.

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https://doi.org/10.1142/S0192415X05003168Cited by:7 (Source: Crossref)

Abstract

Recurrent aphthous ulcerations (RAU) represent a common oral mucosal disease with altered humoral and cellular immunities. Tien-Hsien liquid (THL) is an extract of Chinese medicinal herbs with immunomodulating effects. Our previous study found that THL can modulate the antigen-stimulated proliferative response of peripheral blood mononuclear cells and T-cells isolated from RAU patients. In this study, we further tested whether THL can modulate the antigen-stimulated cytokine production by T-cells isolated from RAU patients. To achieve this goal, T-cells isolated from 19 RAU patients were incubated with phytohemagglutinin (PHA), glutaraldehyde-inactivated tetanus toxoid (TT), glucosyltransferase D (GtfD), or antigens of Streptococcus mutans in the presence or absence of THL. The levels of interleukin (IL)-2, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), IL-6, or IL-10 in the supernatants of T-cell cultures were measured by cytokine enzyme-linked immunosorbent assay (ELISA) kits. We found that THL significantly increased the PHA- or TT-stimulated TNF-α, IL-6, and IL-10 production by T-cells isolated from RAU patients. However, THL could also significantly decrease the TT-stimulated IL-2 production, the GtfD-stimulated IL-2, TNF-α, IL-6 and IL-10 production, and the S. mutans-stimulated IFN-γ, TNF-α, and IL-10 production by T-cells isolated from RAU patients. These results indicate that THL can modulate the antigen-stimulated cytokine production by T-cells isolated from RAU patients. Because RAU is probably a Thl-mediated disease with elevated levels of IL-2, IFN-γ, TNF-α and IL-6 in either the patient's sera or oral lesions and these increased levels of cytokines can be reduced by THL, we suggest that THL may be a potential immunoceutical agent for treatment of RAU.

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