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Differential Gene Expression in Hemodialysis Patients with "Cold" Zheng

Institute of Chinese Medical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C.

Institute of Chinese Pharmaceutical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C.

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Chia-Chang Hsieh

School of Medicine, Kyorin University, Tokyo, Japan, and Jen-Te Junior College of Medicine, Nursing and Management, Miaoli, Taiwan, R.O.C.

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Guang-Wei Chen

Institute of Chinese Medical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C.

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Wen-Huang Peng

Institute of Chinese Pharmaceutical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C.

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, and

Ming-Tsuen Hsieh

Institute of Chinese Pharmaceutical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C.

Correspondence to: Dr. Ming-Tsuen Hsieh, Institute of Chinese Pharmaceutical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, R.O.C. Tel: (+886) 4-2205-3366 (ext. 1706), Fax: (+866) 4-2205-3958.

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https://doi.org/10.1142/S0192415X06003916Cited by:10 (Source: Crossref)

Abstract

The aim of the present study was to search for the differential gene expression and measure the serum level of a number of biochemical parameters in the cold Zheng (CZ) and non-cold Zheng (NCZ) in patients receiving hemodialysis. Hemodialysis (HD) patients were randomly selected from the CZ and NCZ groups. The between-group differences in gene expression were assessed using complementary DNA (cDNA) microarray. Differential gene expression was further validated by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Our results demonstrated that the up-regulation of the inflammation-associated genes, ALOX5AP, S100A8 and S100A12, down-regulation of the genes related to immunity (DEFA4), metabolism (GNG11, PYGB, PRKAR2B), and growth/proliferation (HSF2, DDR2, TK1) were found in the CZ group. Furthermore, the CZ HD patients had significantly lower serum albumin levels compared with their NCZ counterparts (3.31 ± 0.08 g/dL versus 4.18 ± 0.12 g/dL). It appears reasonable to conclude that up-regulated inflammatory-gene expression (ALOX5AP, S100A8 and S100A12) may play an important role in CZ HD patients.

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References

A. R. Amir and J. F. Winchester, Nephrology and Hypertension (Lippincott Williams and Wilkins, Philadelphia, 1999) pp. 236–247. Google Scholar
I. Aoyama, K. Shimokata and T. Niwa, Nephron 90, 297 (2002), DOI: 10.1159/000049066. Crossref, Medline, Web of Science, Google Scholar
R. Carleet al., J. Immunol. 170, 3233 (2003). Medline, Web of Science, Google Scholar
G. T. Chen and S. C. Shiue, Newest Chinese and International Standards for Diagnosis and Treatment (Shyue Yuann Publishing House, Beijing, 1992) pp. 747–749. Google Scholar
J. M. Claverie, Hum. Mol. Genet. 8, 1821 (1999), DOI: 10.1093/hmg/8.10.1821. Crossref, Medline, Web of Science, Google Scholar
B. R. Don and G. Kaysen, Seminars in Dialysis 17(6), 432 (2004), DOI: 10.1111/j.0894-0959.2004.17603.x. Crossref, Medline, Web of Science, Google Scholar
J. S. Guang , The Population of Dialysis in Taiwan ( Publishing House of National Kidney Foundation , Taiwan , 2003 ) . Google Scholar
L. L. Hsiaoet al., Curr. Opin. Nephrol. Hypertens. 9, 253 (2000), DOI: 10.1097/00041552-200005000-00008. Crossref, Medline, Web of Science, Google Scholar
C. H. Hsuet al., Am. J. Chin .Med. 31, 277 (2003), DOI: 10.1142/S0192415X03000965. Link, Web of Science, Google Scholar
T. A. Ikizleret al., Kidney Int. 55, 1945 (1999), DOI: 10.1046/j.1523-1755.1999.00410.x. Crossref, Medline, Web of Science, Google Scholar
E. Imaiet al., Nephrol. Dial. Transplant. 15, 78 (2000), DOI: 10.1093/ndt/15.suppl_6.78. Crossref, Medline, Web of Science, Google Scholar
D. L. Jonathanet al., Kidney Int. 62, 266 (2002), DOI: 10.1046/j.1523-1755.2002.00413.x. Medline, Web of Science, Google Scholar
G. A. Kaysen, J. Am. Soc. Nephrol. 12, 1549 (2001). Crossref, Medline, Web of Science, Google Scholar
C. Kerkhoff, M. Klempt and C. Sorg, Biochim. Biophys. Acta. 1448(2), 200 (1998), DOI: 10.1016/S0167-4889(98)00144-X. Crossref, Medline, Web of Science, Google Scholar
W. Konget al., J. Nan. Uni. TCM. 15, 17 (1999). Web of Science, Google Scholar
G. Lennonet al., Genomics 33, 151 (1996), DOI: 10.1006/geno.1996.0177. Crossref, Medline, Web of Science, Google Scholar
C. M. Luet al., J. Chin. Med. 14, 109 (2003). Google Scholar
A. Monteroet al., J. Nephrol. 16, 682 (2003). Medline, Web of Science, Google Scholar
S. F. Wen, Acta Nephrol. 16, 1 (2002). Medline, Web of Science, Google Scholar
Z. Yanget al., J. Leukocyte Biol. 69(6), 986 (2001). Crossref, Medline, Web of Science, Google Scholar
S. Yuiet al., Mediators Inflamm. 11, 165 (2002), DOI: 10.1155/S0962935102000248. Crossref, Medline, Web of Science, Google Scholar