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Structural Determination and Antioxidant Activity of a Polysaccharide from the Fruiting Bodies of Cultured Cordyceps sinensis

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

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Min Wang

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

Correspondence to: Dr. Min Wang, School/College of Life Science and Technology, China Pharmaceutical University, Tongjia-xiang 24#, 210009, Jiangsu, China. Tel: (+86) 025-8327-1108, Fax: (+86) 025-8327-1249.

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Yun Ling

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

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Weiqiang Fan

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

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Yufeng Wang

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

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Hongping Yin

Department of Microbiology and Biochemistry, School of Life Science and Technology, China Pharmaceutical University, 210009, Jiangsu, China

Correspondence to: Hongping Yin, School/College of Life Science and Technology, China Pharmaceutical University, Tongjia-xiang 24#, 210009, Jiangsu, China. Tel: (+86) 025-8327-1108, Fax: (+86) 025-8327-1249.

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https://doi.org/10.1142/S0192415X09007387Cited by:48 (Source: Crossref)

Abstract

A water-soluble polysaccharide named CPS1 had been isolated from C. sinensis mycelium by hot water extraction, ethanol precipitation, anion-exchange, and gel-permeation chromatography. UV spectra, FTIR spectra, partial acid hydrolysis, PMP precolumn derivation, periodate oxidation and Smith degradation studies were conducted to elucidate its structure. The results indicated that CPS1 was a glucomannogalactan with the monosaccharide composition of glucose: mannose: galactose = 2.8: 2.9: 1. The total carbohydrate content of CPS1 was 99.0%. The weight-average molecular weight was 8.1 × 10³Da. The results predicted (1 → 2) and (1 → 4)-linkage of mannose, (1 → 3)-linkage of galactose, (1 → ) and (1 → 3, 6)-linkage of glucose composed the backbone of CPS1. CPS1 was also evaluated for its antioxidant activity in vitro, including scavenging effects on the hydroxyl radicals, the reducing power, Fe²⁺-chelating activity, scavenging effect on superoxide radicals, as well as the inhibition of hydrogen peroxide induced haemolysis. CPS1 showed a high antioxidant effect, especially scavenging effect of hydroxyl radicals, the reducing power and Fe²⁺-chelating activity. The results provide scientific support for the antioxidant activity and indicated a connection between antioxidant activity and reparation of renal failure.

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