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PI3K/AKT/mTOR Signaling is Involved in (-)-Epigallocatechin-3-Gallate-Induced Apoptosis of Human Pancreatic Carcinoma Cells

Shi Liu

The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China

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Xiao-Jie Wang

Heilongjiang Institute of Dermatology and Sexually Transmitted Disease, Harbin, China

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Ying Liu

The 3rd Affiliated Hospital of Qiqihar Medical College, QiQiHar, China

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, and

Yun-Fu Cui

The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China

Correspondence to: Dr. Yun-Fu Cui, Department of General Surgery, The 2nd Affiliated Hospital, Harbin Medical University, 148 Baojian Road, Harbin, Heilongjiang 150001, China. Tel: (+86) 451-8660-5113.

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https://doi.org/10.1142/S0192415X13500444Cited by:73 (Source: Crossref)

Abstract

PI3K/AKT/mTOR signaling promotes cell survival, proliferation and progression in cancer cells. Targeting this pathway may lead to the development of novel therapeutic approaches for human cancers. Here, we examined the effects of (-)-epigallocatechin-3-gallate (EGCG) on the PI3K/AKT/mTOR pathway in pancreatic cancer cells, and assessed its therapeutic potential. In this study, the proliferation and apoptosis of PANC-1 cells were examined by MTT assay and flow cytometry, respectively. The expression of genes and proteins involved in the PI3K/AKT/mTOR pathway were measured by RT-PCR and western blotting, respectively. Our results revealed that EGCG dramatically inhibited the proliferation of PANC-1 cells and induced apoptosis simultaneously. Furthermore, it upregulated PTEN mRNA and protein expression levels, as well as downregulating the expression of phospho-AKT and phospho-mTOR. In conclusion, these results suggest that EGCG can suppress proliferation and induce apoptosis of PANC-1 cells in a time- and dose-dependent manner; moreover, EGCG also can upregulate PTEN expression and downregulate the expression of pAKT and p-mTOR to modulate the PI3K/AKT/mTOR signaling pathway.

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