Effects of Hachimijiogan, Tokishakuyakusan and Keishibukuryogan on Estrogen and Progesterone Secretions by Rat Preovulatory Follicles

Ovaries of rats 48 hours after injection of 10 IU PMS when 27 days old were incubated or perifused with NIH-LH, Hachimijiogan (TJ-7), Tokishakuyakusan (TJ-23) and Keishibukuryogan (TJ-25).

TJ-7, TJ-23 and TJ-25 decreased estradiol-17 β(E₂) and LH effects on progesterone secretions, while they increased progesterone in media and LH effects on E₂ secretions.

These results suggest that TJ-7, TJ-23 and TJ-25 stimulates preovulatory follicles to secrete progesterone but to suppress E₂ secretions. Furthermore, it is indicated their combination treatment with LH multiplies the sole effect of LH on E₂ secretions but suppresses LH effects on progesterone.