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DISPERSION AND FIXATION OF ADENO-ASSOCIATED VIRUS WITH GLUTARALDEHYDE FOR AFM STUDIES

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China

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,

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China

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, and

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Corresponding author.

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https://doi.org/10.1142/S0218625X08011688Cited by:1 (Source: Crossref)

Abstract

Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%, v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

Keywords:

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Vol. 15, No. 05

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Received 11 January 2008

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