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Regular ArticlesNo Access

VISUALIZATION EX SITU OF SINGLE DNA MOLECULES INCUBATION: A FIRST STEP FOR QUANTITATIVE ANALYSIS ON MULTI-SITE DEGRADATION AND ENZYMATIC KINETICS

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China

Corresponding author.

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China

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https://doi.org/10.1142/S0218625X09012329Cited by:0 (Source: Crossref)

Abstract

Herein, we showed a different approach to directly single-molecule level visualization of the degradation of DNA in vitro tests using DNase I incubation based on high-resolution AFM imaging ex situ with fine relocation nanotechnology. A method of nanomanipulation termed as "modified dynamic molecular combing" (MDMC) was used to pattern DNA samples for further degradation and enzymatic kinetics. This strategy is potentially able to quantitatively address the mechanical-induced kinetic profiles of multi-site degradation of individual DNA molecules with very stable tension and strong immobilization on a surface and discover the mechanochemistry.

Keywords:

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Vol. 16, No. 01

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Received 25 June 2008

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