ELECTROCHEMICAL BIOSENSORS FOR THE DETERMINATION OF GLUTAMATE PYRUVATE TRANSAMINASE AND CREATINE KINASE

Both the determinations of glutamate pyruvate transaminase and creatine kinase were related to low signal detection. Novel amperometric biosensors for the detection of glutamate pyruvate transaminase and creatine kinase were constructed by simple gold two-electrode. The electrode for the determination of GPT was modified with nanoporous platinum-black particles and the electrode for the determination of creatine kinase was modified with porous hydrophilic carboxyl-methyl cellulose. The reagent was added on the electrode surface with nanoporous structure. Ferricyanide was used as electron mediator to detect glutamate pyruvate transaminase. Biosensors appeared good electrochemical characteristics for the rapid measurements of glutamate pyruvate transaminase and creatine kinase, respectively. The sensors showed linear response to aminotransferase and creatine kinase in the concentration range 50–2500 U/L and 50–2000 U/L, showing correlation coefficients 0.991 and 0.995, respectively.