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Effect of disodium N,N-dialanyl protoporphyrinate, diarginine protoporphyrinate and diarginine N,N-dialanyl protoporphyrinate on respiratory burst of human neutrophils in vitro

Andrzej M. Bugaj

University of Health, Beauty and Education, Poznań, Poland

Department of Clinical Pharmacy and Biopharmacy, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

Corresponding author, tel: +48 (61)-8770727, mobile: 501645004.

Current address: Os. Jagielloṅskie 3 m. 9, 61–224 Poznań, Poland.

SPP full member in good standing.

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Hanna Billert

Department of Experimental Anesthesiology, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

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Patrycja Kołodziejska

Department of Clinical Pharmacy and Biopharmacy, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

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Alina Limaszewska

Department of Clinical Pharmacy and Biopharmacy, Karol Marcinkowski University of Medical Sciences, Poznań, Poland

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, and

Zuzanna Stochmal

University of Health, Beauty and Education, Poznań, Poland

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https://doi.org/10.1142/S1088424610001921Cited by:0 (Source: Crossref)

Abstract

The effect of three amino acid protoporphyrin IX (PPIX) derivatives evaluated as sensitizers in photodynamic therapy — disodium N,N-dialanyl protoporphyrinate (PP(Ala)₂Na₂), diarginine protoporphyrinate (PPArg₂) and diarginine N,N-dialanyl protoporphyrinate (PP(Ala)₂Arg₂) – non-irradiated and pre-irradiated with UV-A, on respiratory burst of non-stimulated and opsonized zymosan stimulated neutrophils was studied. A potential synergistic effect of diazepam (7-chloro-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one) was also examined. PP(Ala)₂Na₂ showed strong pro-oxidant effect towards non-stimulated neutrophils, while PPArg₂ and PP(Ala)₂Arg₂ revealed no significant effect. In the case of stimulated neutrophils all studied porphyrins showed antioxidant effect, although for PP(Ala)₂Na₂ this effect was significantly weaker than that of PPArg₂ and PP(Ala)₂Arg₂. After pre-irradiation with UV-A (λ = 365 nm, fluence 2.0 J.cm^-2, fluence rate 6.7 mW.cm^-2) the antioxidant activity of all studied sensitizers towards non-stimulated granulocytes did not change significantly when compared to effects of non-irradiated porphyrins, while in the case of stimulated cells, only PPArg₂ caused significant decrease of respiratory burst. Non-irradiated diazepam showed significant antioxidant effect and enhanced antioxidant effect of all studied porphyrins towards stimulated neutrophils, while after UV-A pre-irradiation it revealed no significant antioxidant effect on non-stimulated and stimulated neutrophils, both alone and in combination with porphyrin sensitizers.

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