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A NOVEL ANTI-ESTROGEN RECEPTOR TARGETED WATER-SOLUBLE QDs FOR BREAST CANCER CELL IMMUNOFLUORESCENT LABELING

Breast Surgical Department of China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130033, P. R. China

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HAILONG HUANG

Alan G. MacDiarmid Institute of Jilin University, 2699 Qianjin Street, Changchun 130012, P. R. China

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JINGYUAN WANG

Alan G. MacDiarmid Institute of Jilin University, 2699 Qianjin Street, Changchun 130012, P. R. China

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GUANG SUN

Breast Surgical Department of China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130033, P. R. China

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, and

ZHELI XU

Breast Surgical Department of China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130033, P. R. China

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https://doi.org/10.1142/S1793292014500738Cited by:1 (Source: Crossref)

Abstract

This research is aimed to develop a novel kind of biomarker based on water-soluble quantum dots (QDs) conjugated with estrogen receptor (ER) for the detection of breast cancer (BC). To achieve the purpose, hydrophobic octadecylamine-coated QDs were encapsulated with amphiphilic biocompatible centipede-like polymer p(aspartate)-Na-graft-p(ethylene glycol)-dodecylamine (PASP-Na-g-PEG-DDA), and then modified with ER monoclonal antibodies, which is one of the most popular biomarkers for the detection and management of breast cancer worldwide. The targeted QDs kept the original optical property of QDs. The cytotoxicity in vitro experiments showed that QDs-ER probes had no obvious toxic side effects. Remarkably, confocal laser scanning microscopy (CLSM) revealed that the localized fluorescence appeared around the nucleus of human breast cancer cell MCF7 (ER positive) compared with that of human breast cancer cell MBA-MD-231 (ER negative). According to the above analysis, this new kind of probe has great potential in the diagnosis of breast tumor in vitro in future clinical application.

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