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Efficient Detection of Biomarker of Radiation-Resistant Nasopharyngeal Carcinoma by Anchor Nucleic Acid Structure and DNase I Amplification

Zhiheng Lu

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

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Yiyang Wu

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

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Qingxiao Wang

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

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Sen Li

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

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Yanfei Cai

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

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, and

Zhaoqi Yang

https://orcid.org/0000-0002-4485-8872

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P. R. China

E-mail Address: zhaoqiyang@jiangnan.edu.cn

Corresponding author.

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https://doi.org/10.1142/S1793292023500133Cited by:0 (Source: Crossref)

Abstract

Radiotherapy is a simple and effective method for the treatment of rhinitis cancer, but some patients are resistant to radiotherapy and affect the curative effect. Previous studies have confirmed that miR-205 can be used as a biomarker for the feasibility of radiotherapy in nasopharyngeal carcinoma (NPC). In this study, a biosensor for the detection of miR-205 was constructed by using graphene oxide (GO) and fluorescent DNA probes, and using DNase I to generate fluorescent signals for cyclic amplification. The results showed that the lowest detection limit of this sensor for detecting miR-205 was 475 pM, which was 4.86 times lower or 4.86 times better than that of conventional methods without amplification, and showed better detection specificity. It is expected to provide a convenient and effective tool for studying the radio resistance mechanism of NPC and for personalized therapy for NPC patients.

Keywords:

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