Research ArticlesOpen Access

In vivo fiber photometry of neural activity in response to optogenetically manipulated inputs in freely moving mice

Liang Li

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Yajie Tang

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Leqiang Sun

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Khaista Rahman

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Kai Huang

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China

Convergence Technology Co. Ltd, 1037 Luoyu Road, Hongshan District, Wuhan 430074, P. R. China

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Weize Xu

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Jinsong Yu

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

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Jinxia Dai

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

Bio-Medical center, Huazhong Agricultural University, Wuhan 430070, P. R. China

E-mail Address: jxdai@mail.hzau.edu.cn

Corresponding authors.

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, and

Gang Cao

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

Bio-Medical center, Huazhong Agricultural University, Wuhan 430070, P. R. China

Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P. R. China

E-mail Address: gcao@mail.hzau.edu.cn

Corresponding authors.

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https://doi.org/10.1142/S1793545817430015Cited by:11 (Source: Crossref)

Abstract

In vivo fiber photometry is a powerful technique to analyze the dynamics of population neurons during functional study of neuroscience. Here, we introduced a detailed protocol for fiber photometry-based calcium recording in freely moving mice, covering from virus injection, fiber stub insertion, optogenetical stimulation to data procurement and analysis. Furthermore, we applied this protocol to explore neuronal activity of mice lateral-posterior (LP) thalamic nucleus in response to optogenetical stimulation of primary visual cortex (V1) neurons, and explore axon clusters activity of optogenetically evoked V1 neurons. Final confirmation of virus-based protein expression in V1 and precise fiber insertion indicated that the surgery procedure of this protocol is reliable for functional calcium recording. The scripts for data analysis and some tips in our protocol are provided in details. Together, this protocol is simple, low-cost, and effective for neuronal activity detection by fiber photometry, which will help neuroscience researchers to carry out functional and behavioral study in vivo.

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References

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Vol. 10, No. 05

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Received 12 February 2017

Accepted 15 March 2017

Published: 21 April 2017

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This is an Open Access article published by World Scientific Publishing Company. It is distributed under the terms of the Creative Commons Attribution 4.0 (CC-BY) License. Further distribution of this work is permitted, provided the original work is properly cited.

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In vivo fiber photometry of neural activity in response to optogenetically manipulated inputs in freely moving mice

Abstract

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