Special Issue on Bio-Inspired Nanotechnology; Guest Editors: Susan Hong Zhou and Yubing Xie — Research ArticlesNo Access

NONBIOFOULING SURFACES COVERED BY BIO-INSPIRED 2-METHACRYLOYLOXYETHYL PHOSPHORYLCHOLINE POLYMER BRUSH BY USE OF POLYMERIC PHOTOINIFERTER

JAMES SIBARANI

Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

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TOMOHIRO KONNO

Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

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MADOKA TAKAI

Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

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, and

KAZUHIKO ISHIHARA

Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

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https://doi.org/10.1142/S1793984412420032Cited by:1 (Source: Crossref)

Abstract

A versatile method for constructing nonbiofouling and micropatterned surfaces bearing brush type poly(2-methacryloyloxyethyl phosphorylcholine) (MPC) on polymeric substrates using living radical polymerization with N,N-diethyldithiocarbamate moiety as a photoiniferter for microbiodevices was demonstrated. The polymeric photoiniferters comprised of 4-vinyl benzyl N,N-diethyldithiocarbamate (VBDC) and 2-ethylhexyl methacrylate (EHMA) were synthesized with variation of VBDC contents from 10% to 40% to easily tune the chain density of poly(MPC) grafted while the chain length was regulated by changing photoirradiation time. The characterizations of the poly(MPC)-modified surfaces were conducted by using attenuated total reflection infrared (ATR-IR), X-ray photoelectron spectroscopy (XPS), ellipsometric measurements, along with atomic force microscopy (AFM). The nonspecific protein adsorption from single and binary solutions of bovine plasma fibrinogen and bovine serum albumin was greatly suppressed on moderate to high chain density, moderate chain length of poly(MPC) and on smooth surfaces. The modified surfaces excellently eliminate cell adhesion of fibroblast-like cell L929 as well. Further, micropatterned-nonbiofouling poly(MPC) brush surface could be constructed using photomask with high fidelity.

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