Special Issue on Nanotoxicology; Guest Editors: G. Sharma and V. KodaliNo Access

Synthesis and Characterization Silver, Zinc Oxide and Hybrid Silver/Zinc Oxide Nanoparticles for Antimicrobial Applications

Myisha Roberson

Department of Materials Science and Engineering, Tuskegee University, Tuskegee, Alabama, USA

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Vijaya Rangari

Department of Materials Science and Engineering, Tuskegee University, Tuskegee, Alabama, USA

Corresponding author.

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Shaik Jeelani

Department of Materials Science and Engineering, Tuskegee University, Tuskegee, Alabama, USA

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Temesgen Samuel

College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama, USA

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, and

Clayton Yates

Department of Biology, Tuskegee University, Tuskegee, Alabama, USA

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https://doi.org/10.1142/S1793984414400030Cited by:34 (Source: Crossref)

Abstract

Silver (Ag) and zinc oxide (ZnO) are well known for both antimicrobial and pro-healing properties. Here, we present a novel method to synthesize Ag and ZnO nanoparticles (NPs), as well as hybrid Ag/ZnO NPs using a custom, temperature controlled microwave assisted technique. Microwave synthesis has been shown not only to enhance the rate of chemical reactions, but also in some cases to give higher product yields over thermal heating. The as-synthesized NPs were characterized by X-ray diffraction (XRD) to study the crystalline structure, composition and purity. Transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) was used to study particle size, shape, composition and morphology. These results indicated that the as-prepared Ag NPs are spherical in shape and ~ 20 nm in sizes. The ZnO NPs are typically rod shaped and the particle sizes are ~ 20 nm in width and 100 nm in length. These NPs were tested for antibacterial and/or antifungal properties using disc diffusion assays. Results show microwave synthesized NPs inhibit growth of S. aureus, E. coli and C. albicans at 50 μ g/mL treatment concentration. Ag NPs were most effective in inhibiting bacterial and fungal growth at the concentrations tested followed by hybrid Ag/ZnO and ZnO nanoparticles. These results also suggest that the hybridization of ZnO to Ag NPs may reduce the toxicity of Ag NPs. Further studies are needed to understand the functional interaction between the two types of NPs and to improve their ability for biological or biomedical application.

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References

M. Pautler, Int. J. Nanomed. 5, 803 (2010). Google Scholar
W. E. Bawarski, Nanomedicine 2, 273 (2008). Google Scholar
H. Nalwa , Nanostructured Materials and Nanotechnology ( Academic Press , USA , 2002 ) . Google Scholar
S. Ostrovsky, Nano Res. 2, 882 (2009), DOI: 10.1007/s12274-009-9089-5. Crossref, Google Scholar
J. Pechère, Clinical Infectious Diseases 33, S170 (2001). Crossref, Google Scholar
E. Roston, PubMed: http://www.ncbi.nlm.nih.gov/pubmed/12358835 (2009) . Google Scholar
A. Shahverdi, Nanomed. Nanotechnol., Biol. Med. 3, 168 (2007), DOI: 10.1016/j.nano.2007.02.001. Crossref, Google Scholar
M. Fang, Int. J. Antimicrobial Agents 27, 513 (2006), DOI: 10.1016/j.ijantimicag.2006.01.008. Crossref, Google Scholar
M. Liong, ACS Nano 2, 889 (2008), DOI: 10.1021/nn800072t. Crossref, Google Scholar
P. Dibrov, Antimicrobial Agents Chemotherapy 46, 2668 (2002), DOI: 10.1128/AAC.46.8.2668-2670.2002. Crossref, Google Scholar
V. K. Rangariet al., J. Crystal Growth 250, 409 (2003). Google Scholar
E. Shameli, Int. J. Nanomed. 5, 573 (2010). Google Scholar
E. Shameli, Int. J. Nanomed. 6, 331 (2011). Google Scholar
I. Sondi, J. Colloid Interface Sci. 275, 177 (2004), DOI: 10.1016/j.jcis.2004.02.012. Crossref, Google Scholar
K. Dhermendraet al., Current Sci. 95, 647 (2008). Google Scholar
J. S. Kim, Nanomed. 3, 95 (2007), DOI: 10.1016/j.nano.2006.12.001. Crossref, Google Scholar
H. H. Lara, J. Nanobiotechnol. 9, 30 (2011), DOI: 10.1186/1477-3155-9-30. Crossref, Google Scholar
Y. Xiao-Yun, J. Nanoparticle Resolution 11, 1159 (2009). Google Scholar
Y. Zhang, J. Colloid Interface Sci. 301, 478 (2007). Google Scholar
G. M. Neelgund, J. Nanosc. Nanotechnol. 3621 (2011), DOI: 10.1166/jnn.2011.3756. Google Scholar
P. Mariappan, Nanomed.: Nanotechnol. Biol. Med. 7, 184 (2011). Google Scholar
W. Rizwan, J Nanoscale Resolution Lett. 5, 1675 (2010). Google Scholar
M. J. Heightet al., Appl. Catalysis B 63, 305 (2006), DOI: 10.1016/j.apcatb.2005.10.018. Crossref, Google Scholar
F. E. Bensebaa, J. Mater. Chem. 14, 3378 (2004), DOI: 10.1039/b404280h. Crossref, Google Scholar
B. L. Hayes , Microwave Synthesis: Chemistry at the Speed of Light ( CEM Publishing , Mathews , 2002 ) . Google Scholar
K. Oliver , Practical Microwave Synthesis for Organic Chemists Strategies, Instruments, and Protocols ( Verlag GmbH & Co , Weinheim , 2009 ) . Google Scholar
C. O. Kappe, Angew Chem. Int. Ed. 43, 6250 (2004), DOI: 10.1002/anie.200400655. Crossref, Google Scholar
V. K. Rangari, Mater. Sci. Eng.: B 168, 117 (2010), DOI: 10.1016/j.mseb.2010.01.013. Crossref, Google Scholar
G. Maioranoet al., ACS Nano 4, 7481 (2010), DOI: 10.1021/nn101557e. Crossref, Google Scholar
M. Palvin and V. Bregar, Digest J. Nanomater. Biostruct. 7, 1389 (2012). Google Scholar
Z. Jiet al., Environ. Sci. Technol. 44, 7309 (2010), DOI: 10.1021/es100417s. Crossref, Google Scholar
J. Hudzicki, ASM Microbe Lib. (Washington, DC, 2009), www.microbelibrary.org, accessed 1 December 2012 . Google Scholar
O. Yamamoto, J. Ceramic Soc. Japan 106, 1007 (1999), DOI: 10.2109/jcersj.106.1007. Crossref, Google Scholar