Research ArticlesOpen Access

Influence of Cholecystokinin-8 on Compound Nerve Action Potentials from Ventral Gastric Vagus in Rats

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

E-mail Address: k.mirza@imperial.ac.uk

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Andrea Alenda

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

E-mail Address: a.alenda@imperial.ac.uk

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Amir Eftekhar

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

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Nir Grossman

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

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Konstantin Nikolic

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

E-mail Address: k.nikolic@imperial.ac.uk

Corresponding author.

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Stephen R. Bloom

Division of Diabetes, Endocrinology and Metabolism, Section of Endocrinology and Investigative Medicine, Imperial College London, W12 0NN, UK

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, and

Christofer Toumazou

Institute of Biomedical Engineering, Department of Electrical and Electronic Engineering, Imperial College London, SW7 2AZ, UK

E-mail Address: c.toumazou@imperial.ac.uk

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https://doi.org/10.1142/S0129065718500065Cited by:8 (Source: Crossref)

Abstract

Objective: Vagus Nerve Stimulation (VNS) has shown great promise as a potential therapy for a number of conditions, such as epilepsy, depression and for Neurometabolic Therapies, especially for treating obesity. The objective of this study was to characterize the left ventral subdiaphragmatic gastric trunk of vagus nerve (SubDiaGVN) and to analyze the influence of intravenous injection of gut hormone cholecystokinin octapeptide (CCK-8) on compound nerve action potential (CNAP) observed on the same branch, with the aim of understanding the impact of hormones on VNS and incorporating the methods and results into closed loop implant design. Methods: The cervical region of the left vagus nerve (CerVN) of male Wistar rats was stimulated with electric current and the elicited CNAPs were recorded on the SubDiaGVN under four different conditions: Control (no injection), Saline, CCK1 (100pmol/kg) and CCK2 (1000pmol/kg) injections. Results: We identified the presence of A $δ$ $δ$ , B, C1, C2, C3 and C4 fibers with their respective velocity ranges. Intravenous administration of CCK in vivo results in selective, statistically significant reduction of CNAP components originating from A and B fibers, but with no discernible effect on the C fibers in $n = 7$ $n = 7$ animals. The affected CNAP components exhibit statistically significant ( $p_{Saline - CCK 1} = 0.02$ $p_{Saline - CCK 1} = 0.02$ and $p_{Saline - CCK 2} = 0.007$ $p_{Saline - CCK 2} = 0.007$ ) higher normalized stimulation thresholds. Conclusion: This approach of characterizing the vagus nerve can be used in closed loop systems to determine when to initiate VNS and also to tune the stimulation dose, which is patient-specific and changes over time.

Keywords:

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Vol. 28, No. 09

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Accepted 4 February 2018

Published: 9 April 2018

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This is an Open Access article published by World Scientific Publishing Company. It is distributed under the terms of the Creative Commons Attribution 4.0 (CC-BY) License. Further distribution of this work is permitted, provided the original work is properly cited.

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Influence of Cholecystokinin-8 on Compound Nerve Action Potentials from Ventral Gastric Vagus in Rats

Abstract

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