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POSTER PRESENTATION: EmbryologyOpen Access

#136 : Osteopontin Facilitates Embryo Growth, Implantation, and Cellular Metabolism via Interacting with αvβ3 and RGS2 Pathway: Evidence from Mouse Embryos and a 3D In-Vitro Implantation Model

    https://doi.org/10.1142/S2661318223744181Cited by:0 (Source: Crossref)
    This article is part of the issue:

    Background and Aims: We conducted a study on mice to investigate how osteopontin, a mediator of cell-cell and cell-extracellular matrix (ECM) communication and invasion, influences embryonic development, metabolism, and implantation potential, with confirmation by a 3D in-vitro implantation model.

    Method: Embryos were collected from female mice and cultured under different conditions: HTF only, HTF supplemented with OPN at different doses, and OPN-supplemented HTF with anti-αvβ3 Ab or RGS2 inhibitors. Embryo morphology, development, and implantation rates were evaluated. Microarrays were used to evaluate gene expression profiling. Choriocarcinoma JAR and epithelial RL95-2 cell lines were adopted for the in-vitro implantation model. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were used to measure cellular metabolism levels.

    Results: The supplementation of OPN best enhanced the rate of embryo hatching-out and implantation onto the matrigel at 100 nM, but a higher degeneration rate was seen at a higher concentration (200 nM). In OPN-treated embryos, microarray analysis showed 51 significantly down-regulated genes and 60 up-regulated genes, including RGS2. Inhibition of αvβ3 integrin decreased embryo implantation rates and down-regulated RGS2. Validation of the 3D implantation model using JAR spheroids and RL95-2 monolayer showed a similar effect. OPN also increased OCR and ECAR of the JAR cells, and migration assays revealed increased migration ability in OPN-overexpressed JAR cells, which was then inhibited by αvβ3 and RGS2 inhibitors.

    Conclusion: Osteopontin, at a reasonable concentration, could enhance embryo development and implantation by interacting with αvβ3 integrin, subsequently facilitating the RGS2 pathway. OPN has the potential to be a supplement during in vitro embryo culture in ART intervention.

    Publisher's Note:

    This article contains the abstract sections only.