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THE BEHAVIOR OF RAT TOOTH GERM CELLS ON 3-HYDROXYL-BUTYRATE-CO-3-HYDROXY-HEXANOATE (PHBHHx) MEMBRANES

Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan

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Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan

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Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 100, Taiwan

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Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan

School of Dentistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan

Corresponding author: Dr Min-Huey Chen, School of Dentistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan.

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Tai- Horng Young

Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan

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https://doi.org/10.4015/S1016237207000379Cited by:0 (Source: Crossref)

Abstract

Copolymers of 3-hydroxyvalerate (HV) and 3-hydroxyhexanoate (HHx) membranes are a new family of biomaterials for tissue engineering applications. The object of this study is to investigate the behavior of rat tooth germ cells on various 3-hydroxyl-butyrate-co-3-hydroxy-hexanoate (PHBHHx) membranes. In this study, PHBHHx membranes from three thin-film processes were used. PHBHHx membranes with different surface morphologies were prepared by phase inversion, electrospinning, and hot pressing. The morphologies of the PHBHHx membranes were investigated by scanning electron microscopy (SEM). Tooth germ cells were isolated from four-day-old Wistar rats. The cellular adhesion, proliferation and viability were determined by SEM, BrdU (5-bromo-2-deoxyuridine) and MTT (3-[4, 5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue) assay. In addition, the adherent behavior of tooth germ cells on various surface structures of PHBHHx was observed under a fluorescence microscope after staining of the cytoskeletal filamentous actin of the cells. It was found that cell compatibility of the PHBHHx membranes made from the phase inversion method (p-PHBHHx) was better than that of the other PHBHHx membranes. The results also revealed that tooth germ cells cultured on the PHBHHx membranes with porous surface structure were well spread relative to those on the fibrous structure of PHBHHx membranes. Therefore, PHBHHx membranes with a porous surface structure can encourage either cell adhesion or cell proliferation. PHBHHx membranes with a porous morphology satisfy biomaterial requirements for a scaffold for tooth regeneration.

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References

G. Q. Chen and Q. Wu, Biomaterials 26, 6565 (2005), DOI: 10.1016/j.biomaterials.2005.04.036. Crossref, Web of Science, Google Scholar
S. Khanna and A. K. Srivastava, Proc. Biochem. 40, 607 (2005), DOI: 10.1016/j.procbio.2004.01.053. Crossref, Web of Science, Google Scholar
N. Yoshie, M. Saito and Y. Inoue, Macromolecules 34, 8953 (2001), DOI: 10.1021/ma0113071. Crossref, Web of Science, Google Scholar
X. H. Quet al., Biomaterials 27, 2944 (2006), DOI: 10.1016/j.biomaterials.2006.01.013. Crossref, Web of Science, Google Scholar
K. Zhao, Y. Deng and G.-Q. Chen, Biochem. Eng. J. 16, 115 (2003). Web of Science, Google Scholar
K. Zhaoet al., Biomaterials 24, 1041 (2003), DOI: 10.1016/S0142-9612(02)00426-X. Crossref, Web of Science, Google Scholar
J. Liet al., J. Biomed. Mater. Res. A 75, 985 (2005). Web of Science, Google Scholar
J. Xiet al., J. Biomater. Sci. Polym. Ed. 16, 1395 (2005), DOI: 10.1163/156856205774472344. Crossref, Web of Science, Google Scholar
S. Chenget al., Biomaterials 27, 3758 (2006), DOI: 10.1016/j.biomaterials.2006.02.046. Crossref, Web of Science, Google Scholar
W. Y. Chuanget al., Biomaterials 20, 1479 (1999), DOI: 10.1016/S0142-9612(99)00054-X. Crossref, Web of Science, Google Scholar
B. Duanet al., J. Biomater. Sci. Polym. Ed. 18, 95 (2007), DOI: 10.1163/156856207779146105. Crossref, Web of Science, Google Scholar
T. G. Kim and T. G. Park, Tissue Eng. 12, 221 (2006), DOI: 10.1089/ten.2006.12.221. Crossref, Google Scholar
M. A. Pattisonet al., Biomaterials 26, 2491 (2005), DOI: 10.1016/j.biomaterials.2004.07.011. Crossref, Web of Science, Google Scholar
L. A. Hidalgo-Bastidaet al., Acta. Biomater. 3, 457 (2007). Crossref, Web of Science, Google Scholar
D. T. Lin, T. H. Young and Y. Fang, Biomaterials 22, 1521 (2001), DOI: 10.1016/S0142-9612(00)00308-2. Crossref, Web of Science, Google Scholar
S. A. C. Modino and P. T. Sharpe, Arch. of Oral. Biol. 50, 255 (2005), DOI: 10.1016/j.archoralbio.2005.01.002. Crossref, Web of Science, Google Scholar
C. S. Younget al., J. Dent. Res. 81, 695 (2002). Crossref, Web of Science, Google Scholar
Y. W. Wang, Q. Wu and G. Q. Chen, Biomaterials 25, 669 (2004), DOI: 10.1016/S0142-9612(03)00561-1. Crossref, Web of Science, Google Scholar
W. Juan, Y. Zhiming and C. Tianquan, Frontiers of Forestry in China 3, 343 (2006). Google Scholar
J. S. Choiet al., Int. J. Biol. Macromol. 34, 249 (2004), DOI: 10.1016/j.ijbiomac.2004.06.001. Crossref, Web of Science, Google Scholar
D. Ishiiet al., J. Biotechnol. (2007). Google Scholar
T. H. Younget al., Biomaterials 19, 717 (1998), DOI: 10.1016/S0142-9612(98)00187-2. Crossref, Web of Science, Google Scholar
M. H. Chenet al., J. Biomed. Mater. Res. A 74, 254 (2005). Google Scholar
T. H. Young and C. H. Hung, Biomaterials 26, 4291 (2005), DOI: 10.1016/j.biomaterials.2004.10.043. Crossref, Web of Science, Google Scholar
M. H. Chenet al., Tissue Eng. 11, 526 (2005), DOI: 10.1089/ten.2005.11.526. Crossref, Google Scholar
N. Kusunokiet al., BMC Pharmacol. 4, 2 (2004), DOI: 10.1186/1471-2210-4-2. Crossref, Google Scholar
B. I. Tarnowski, F. G. Spinale and J. H. Nicholson, Biotech. Histochem. 66, 297 (1991). Google Scholar
T. Okanoet al., Biomaterials 16, 297 (1995), DOI: 10.1016/0142-9612(95)93257-E. Crossref, Web of Science, Google Scholar
J. E. Mitchellet al., J. Biol. Chem. 281, 5718 (2006), DOI: 10.1074/jbc.M509775200. Crossref, Web of Science, Google Scholar
C. S. Chenet al., Science 276, 1425 (1997), DOI: 10.1126/science.276.5317.1425. Crossref, Web of Science, Google Scholar
R. Rajaramanet al., Exp. Cell Res. 88, 327 (1974), DOI: 10.1016/0014-4827(74)90248-1. Crossref, Web of Science, Google Scholar
B. Albertset al., Mol. Biol. of the Cell (1994). Google Scholar
J. Y. Lim et al. , J. R. Soc. Interface 2 , 97 . Crossref, Web of Science, Google Scholar

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Vol. 19, No. 05

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Accepted 23 October 2007

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