USING DIT-FFT ALGORITHM FOR IDENTIFICATION OF PROTEIN CODING REGION IN EUKARYOTIC GENE
Abstract
The new research platform on biomedical engineering by Digital Signal Processing (DSP) is playing a vital role in the prediction of protein coding regions (Exons) from genomic sequences with great accuracy. We can determine the protein coding area in DNA sequences with the help of period-3 property. It has been seen that in order to find out the period-3 property, the DFT algorithm is mostly used but in this paper, we have tested FFT algorithm instead of DFT algorithm. DSP is basically concerned with processing numerical sequences. When digital signal processing used in DNA sequences analysis, it requires conversion of base characters sequence to the numerical version. The numerical representation of DNA sequences strongly impacts the biological properties mirrored through the numerical genre. In this work, the proposed technique based on DIT-FFT algorithm has been used to identify the exonic area with the help of integer value representation for transforming the DNA sequences. Digital filters are used to read out period 3 components from the output spectrum and to eliminate the unwanted high frequency noise from DNA sequences. To overcome background noise means to suppress the non-coding regions, i.e., Introns. Proposed algorithm is tested on four nucleotide sequences having single or multiple numbers of exons.
