Protein S-Thiolation, S-Nitrosylation, and Irreversible Sulfhydryl Oxidation: Roles in Redox Regulation

The following sections are included:

• Summary

  • Roles of protein cysteines in cells

  • Molecular Mechanisms of S-thiolation and S-nitrosylation

  • Molecular Mechnism of Dethiolation and Denitrosylation

  • Effects of Oxidative Modification on Protein Function

• Analytical Methods for Protein Oxidation

  • Protein Separation by Charge

• Oxidative Modification of Reactive Cysteines in Selected Proteins

  • Abundant Proteins

  • Less Abundant Proteins

• Perspective — Questions in Need of Answers

• Acknowledgment

• References