World Scientific
Skip main navigation

Cookies Notification

We use cookies on this site to enhance your user experience. By continuing to browse the site, you consent to the use of our cookies. Learn More
×

System Upgrade on Tue, May 28th, 2024 at 2am (EDT)

Existing users will be able to log into the site and access content. However, E-commerce and registration of new users may not be available for up to 12 hours.
For online purchase, please visit us again. Contact us at customercare@wspc.com for any enquiries.

Chapter 7: Cell-Based Biosensors

    https://doi.org/10.1142/9789811238031_0007Cited by:1 (Source: Crossref)
    Abstract:

    Living cells are considered to be biorecognition elements that have been extensively utilized in the field of biosensors since the early 1970s, especially in biological and electronics applications. They are acting as suitable bioreceptor elements because they offer flexibility to sensing strategies, and make the fabrication process relatively simple and cheap compared to pure enzymes, DNA and antibodies. In the last five years, there was a massive advancement in the construction of cell-based biosensors (CBBs) and their application in many fields. Recently, 3D electrode-based cell culture platforms were fabricated to provide new information about the pathological and physiological features of the disease, while a cardiomyocyte-integrated microelectrode array (MEA) was developed for the evaluation of the cardiac toxicity caused by the drugs. Additionally, the plasmonic devices were fabricated for anti-microbial susceptibility testing. What’s more, the discovering of microbial fuel cell biosensors permits the specific determination of important analytes and also acting as multiparametric devices for real-time assessment and monitoring of more than one cell parameter. Nevertheless, problems such as cell populations heterogeneity, storage lives and regeneration, unselectively and costs of instruments must be taken into consideration prior to full fabrication of these CBBs. This chapter summarizes the types of cells used for the construction of CBBs and their immobilization techniques on different transducers. Also, we summarize the results of the previously reported papers about the different applications of CBBs.