Differentiation of Panax ginseng from P. quinquefolius by Direct Amplification of Length Polymorphisms

The following sections are included:

• General Principles of DALP for Authentication

• Materials and Experimental Methods

  • Materials

  • Experimental Methods

    • DNA extraction

    • Choice of primers

    • Alternative labelled amplification reaction

    • Electrophoresis of the multi-locus amplification products

    • DNA elution from dried sequencing gel

    • Re-amplification of polymorphic DALP fragments from dried sequencing gel

    • Conversion of DALP marker to Sequence Tagged Site (STS) marker

• Results

• Discussion

• Pros and Cons of Using the DALP Approach

• References