VISUALIZATION OF FLAVOR RELEASE IN THE SPRAY-DRIED PARTICLE BY CONFOCAL LASER SCANNING MICROSCOPY

The release of d-limonene from a single spray-dried particle could be measured with CLSM through the change in fluorescence intensity of Nile red. Fluorescence change of Nile red indicated that d-limonene release from the spray-dried particle containing emulsified d-limonene occurred from the inner part of the spray-dried particle. The change in fluorescence intensity was similar to the d-limonene content in the spray-dried powder measured by gas chromatography. These results suggest that CLSM could possibly serve as a useful means for observation of flavor release behavior in spray-dried particles.