THREE-PHASE PARTITIONING (TPP): A RAPID AND PREPARATIVE PURIFICATION TOOL FOR GFP

In the current era of proteomics and high throughput drug screening, rapid protein purification methods that do not compromise purity, yield, and sample volume are in great demand. We present a three-phase partitioning method which is highly applicable to the bioluminescent reporter protein GFP (green fluorescent protein).^1,2 Purifications in excess of 100-fold from whole, unlysed bacterial cells is achieved with nearly full GFP recovery in less than 10 minutes (on a micro scale). The method is highly scaleable, performed at a macro level in a matter of several hours…