INTRON-SIZE AND EXON POLYMORPHISMS IN THE MOUSE TISSUE-NONSPECIFIC ALKALINE PHOSPHATASE GENE

Restriction analysis of genomic DNA from five mouse strains revealed previously unknown variations of the mouse TNAP gene, i.e. point mutations and intron-size differences. Our cloning and sequencing of 17.5 kb of the Balb/c TNAP gene allowed for a detailed analysis of these polymorphisms. Comparing the exonic sequences with previously published cDNAs, we found amino acid substitutions compatible with protein polymorphisms observed electrophoretically. Most variations, however, are due to intronic sequence polymorphisms, including variable length simple sequence repeats.