EXPERIMENTAL PHARMACOLOGICAL RESEARCHES CONCERNING VEGETAL EXTRACTS IN HIGH DILUTIONS: II. BELLADONNA, AT VERY WIDE SCALE OF DILUTIONS, IN VITRO, ON THE ISOLATED RAT DUODENUM
The Belladonna (B) tincture (containing 0.03 g% total alkaloids, expressed in atropine) was progressively diluted with water, at a geometrical ratio of 1: 100. Thus, a scale of centesimal dilutions (C) between the 1st dilution 10−1 (1C) and the 200th dilutions 10−400 (200 C), with a content of 6.023 × 1017 and respectively 6.023 × 10”381 atropine molecules/100 ml, was obtained. The dilutions were prepared in two ways: a) by simple homogenization (N dilutions) and b) by “dynamization” with manual succussion according to Hahnemann's homeopathic technique (D dilutions). The in vitro classical pharmacodynamic technique, on the isolated rat duodenum, mounted in the isolated organ bath, with special precautions was used. The isotonic contraction was recorded. The effects on the spasm induced by acetylcholine (ACH), at the standard dose of 10−6 g/ml bath, in the aqueous N solution, with a content of 33.55 − 1018 ACH molecules/100 ml was studied. On the scale of D dilutions 1C - 200C of B, the effect had a sinusoidal course (bidirectional multiphase effect). In the light of the author's informational theory of dosages, this double way (stimulator - inhibitor) effect is possible at different value scales of concentrations. In accordance with this theory, the information of biological and drug signals come out, may change and diversify, not only from the nature of substance and energy and also from their order and organisation in space and time, but also with their quantity, within certain limits. The defmite organisation of a solvent as the specific informational substrate of very high D dilutions of B was investigated, by a calorimetric methodology.
