Biomolecular Computing Realized in Parallel Flow Systems: Enzyme-Based Double Feynman Logic Gate

An enzyme system organized in a flow device with three parallel channels was used to mimic a reversible Double Feynman Gate (DFG) with three input and three output signals. Reversible conversion of NAD⁺ and NADH cofactors was used to perform XOR logic operations, while biocatalytic oxidation of NADH resulted in Identity operation working in parallel. The first biomolecular realization of a DFG gate is promising for integrating into complex biomolecular networks operating in future unconventional biocomputing systems, as well as for novel biosensor applications.