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Estrogen Activities and the Cellular Effects of Natural Progesterone from Wild Yam Extract in MCF-7 Human Breast Cancer Cells

Department of Bioscience and Biotechnolgy, College of Engineering, Institute of Biotechnology, Sejong University, Seoul, Korea

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Hyeok-Yi Kwon

Department of Bioscience and Biotechnolgy, College of Engineering, Institute of Biotechnology, Sejong University, Seoul, Korea

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Woong-Shick Ahn

Department of Obstetrics and Gynecology, The Catholic University of Korea College of Medicine, Seoul, Korea

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Sumi Bae

Department of Obstetrics and Gynecology, The Catholic University of Korea College of Medicine, Seoul, Korea

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Mee-Ra Rhyu

Food Function Research Division, Korea Food Research Institute, Gyeonggi-Do 463-476, Korea

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, and

YoungJoo Lee

Department of Bioscience and Biotechnolgy, College of Engineering, Institute of Biotechnology, Sejong University, Seoul, Korea

Correspondence to: Dr. Young Joo Lee, Department of Bioscience and Biotechnology, Sejong University, Kwang-Jin-Gu, Seoul, Korea. Tel: (+82) 2-3408-3640, Fax: (+82) 2-3408-3334.

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https://doi.org/10.1142/S0192415X09006746Cited by:18 (Source: Crossref)

Abstract

We studied the estrogenic activity and cellular effect of wild yam extract in MCF-7 human breast cancer cells. The extract increased the activity of the progesterone receptor and pS2 genes at the mRNA levels in human breast cancer MCF-7 cells, although the effects were not as prominent as those of 17β-estradiol (E₂). Western blot analysis showed that the level of estrogen receptor α protein was down-regulated after treatment with E₂ or wild yam extract. Wild yam extract also inhibited proliferation of MCF-7 cells. These data indicate that wild yam extract acts as a weak phytoestrogen and protects against proliferation in human breast carcinoma MCF-7 cells.

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