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Selective hydroxylation of naphthalene using the H₂O₂-dependent engineered P450BM3 driven by dual-functional small molecules

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, Hubei, China

Shandong Provincial Key Laboratory of Synthetic Biology, and CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China

These authors contributed equally to this work.

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Jie Chen

University of Chinese Academy of Sciences, Beijing 100049, China

These authors contributed equally to this work.

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Nana Ma

University of Chinese Academy of Sciences, Beijing 100049, China

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Haifeng Zhou

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, Hubei, China

E-mail Address: zhouhf@ctgu.edu.cn

Correspondence to: Haifeng Zhou, tel.: +86-717-6393235

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, and

Zhiqi Cong

E-mail Address: congzq@qibebt.ac.cn

Correspondence to: Zhiqi Cong, tel.: +86-532-8066-2758

SPP full member in good standing

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https://doi.org/10.1142/S108842461850061XCited by:19 (Source: Crossref)

Abstract

We herein report the H₂O₂-dependent selective hydroxylation of naphthalene catalyzed by engineered P450BM3 with the assistance of dual-functional small molecules (DFSMs). The mutation at position 268 significantly improved the hydroxylation activity of P450BM3, which is quite different from those engineered P450BM3 peroxygenases and NADPH-dependent P450BM3 mutants previously reported, implicating the unique role of the residue at position 268. This study provides a potential approach to develop the practical hydroxylation biocatalyst of P450s for aromatic hydrocarbons using the DFSM-facilitated P450BM3-H₂O₂ system.

Dedicated to Professor Naisheng Chen on the occasion of his 80th birthday

Keywords:

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