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Preparation of QDs@SiO₂/Polystyrene Composite Particles for Cancer Cells Detection

School of Materials Science and Engineering, Tongji University, Shanghai 200092, P. R. China

The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200092, P. R. China

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Yilong Wang

The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200092, P. R. China

E-mail Address: yilongwang@tongji.edu.cn

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Donglu Shi

The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200092, P. R. China

The Materials Science and Engineering Program, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA

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https://doi.org/10.1142/S1793984418410064Cited by:3 (Source: Crossref)

This article is part of the issue:

Special Issue on Stem Cells and Nanomaterials for Regenerative Medicine; Guest Editors: Zhongmin Liu, Qing Liu and Wenjie Tang

Abstract

We report a sandwich immunoassay strategy for specific isolation and detection of cancer cells. Prostate cancer LNCaP cells overexpressing PSMA (Prostate-specific membrane antigen) were used as the model target to evaluate the performance of immunoassay. Inorganic/polymer nanocomposites with Janus-like structure and enhanced fluorescence properties were synthesized and applied for immobilization of QDs and the antibodies. PSMA antibodies were covalently conjugated to the QDs@SiO₂/PS Janus-like composite nanoparticles to construct the fluorescent probes. Magnetic probes conjugated with polyclonal antibodies were used to capture and isolate the cancer cells. Results indicated high potential of the unique nanoprobes in specific isolation and detection of cancer cells.

Keywords:

References

1. N. Joumaa, M. Lansalot, A. Theretz and A Elaissari, Langmuir 22, 1810 (2006). Crossref, Google Scholar
2. M. Chen and M. Yin, Prog. Polym. Sci. 39, 365 (2014). Crossref, Google Scholar
3. C. J. Murphy, Anal. Chem. 74, 520A (2002). Crossref, Google Scholar
4. J. H. Warner, A. Hoshino, K. Yamamoto and R. D. Tilley, Angew. Chem. Int. Ed. 44, 4550 (2005). Crossref, Google Scholar
5. M. Rosso-Vasic, E. Spruijt, Z. Popovic, K. Overgaag, B. van Lagen, B Grandidier, D. Vanmaekelbergh, D. Dominguez-Gutierrez, L. De Cola and H. Zuilhof, J. Mater. Chem. 19, 5926 (2009). Crossref, Google Scholar
6. C. Zhou, M. Mao, H. Yuan, H. Shen, F. Wu, L. Ma and L. S. Li, J. Nanoparticle Res. 15, 1901 (2013). Crossref, Google Scholar
7. J. G. Zhang, N. Coombs and E. Kumacheva, J. Am. Chem. Soc. 124, 14512 (2002). Crossref, Google Scholar
8. J. G. Zhang, N, Coombs, E. Kumacheva, Y. K. Lin and E. H. Sargent, Adv. Mater. 14, 1756 (2002). Crossref, Google Scholar
9. A. C. C. Esteves, L. Bombalski, T. Trindade, K. Matyjaszewski and A. Barros-Timmons, Small 3, 1230 (2007). Crossref, Google Scholar
10. J. Qian, C. Zhang, X. Cao and S. Liu, Anal. Chem. 82, 6422 (2010). Crossref, Google Scholar
11. F. Wang, G. M. Pauletti, J. Wang, J. Zhang, R. C. Ewing, Y. Wang and D. Shi, Adv. Mater. 25, 3485 (2013). Crossref, Google Scholar
12. S. S. Banerjee, A. Jalota-Badhwar, S. D. Satavalekar, S. G. Bhansali, N. D. Aher, R. R. Mascarenhas, D. Paul, S. Sharma and J. J. Khandare, Adv. Healthc. Mater. 2, 800 (2013). Crossref, Google Scholar
13. H. Yang, L. Qin, Y. Wang, B. Zhang, Z. Liu, H. Ma, J. Lu, X. Huang, D. Shi and Z. Hu, Int. J. Nanomed. 10, 77 (2015). Google Scholar