Gait assessment is important for identification of potential faller among the elderly populations. Slope walking is associated with fall risk factor and elderly women have higher fall rate compared with elderly men. Therefore, this study investigated gait characteristics of elderly women in overground and slope walkway conditions. Thirty healthy elderly women (15 younger-elderly women and 15 older-elderly women) walked along the linear walkway including three walking conditions (overground, ascent and descent conditions). Temporal gait variables and normalized peak vertical GRF (ground reaction force) variables were derived from commercial motion analysis software. Repeated-measures analysis of variance (ANOVA) was evaluated to compare mean differences of the three conditions and mean difference between younger and older elderly women. All gait characteristics were significantly different from the slope walking conditions (p<0.05). Elderly women walked with longer loading response and mid stance phase during descent walking. Also, ascent walking induced a longer terminal stance phase. Interactions of age and walkway conditions were also significant in vertical GRF, where older-elderly women were greater than younger-elderly women in ascent walkway condition (p<0.01) and in descent walkway condition (p=0.05). These findings suggest that specific-walkway condition should be considered for fall prevention and clinical interventions in elderly women.