The orthogonality graphO(R) of a ring R is the undirected graph with vertex set consisting of all nonzero two-sided zero divisors of R, in which for two vertices x and y (needless distinct), x∼y is an edge if and only if xy=yx=0. Let n≥2, Matn(F) be the set of all n×n matrices over a finite field F, and Rn(F) the subset of Matn(F) consisting of all rank one upper triangular matrices. In this paper, we describe the full automorphism group, and using the technique of generalized equivalent canonical form of matrices, we compute the fixing number of O(Rn(F)), the induced subgraph of O(Matn(F)) with vertex set Rn(F).