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Graphene oxide nanosheet is an ideal platform to capture nanoparticles for highly efficient catalysis, electrochemical sensing and biosensing. In this work, we have described a simple synthesis method for preparation graphene oxide–Au nanohybrid. Au nanodots with an average size of 1.6 nm uniformly dispersed on the surface of graphene oxide. The well-defined nanostructure has been characterized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). The nanohybrid also exhibits enhanced catalytic activity toward the reduction of 4-nitrophenol by NaBH4. Comparing with pure Au nanodots and graphene oxide, graphene oxide–Au nanohybrid shows the highest catalytic activity. This approach not only suggests a wide potential application of graphene oxide nanosheet as a host material for supporting a variety of nanoparticles, but also provides a new approach for the fabrication of graphene-based nanohybrids with multiple physical and chemical properties.