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chapterNo Access
Changes in microbial population affected by physico-chemical conditions of soils contaminated by explosives
- B. Limane,
- J. Juhanson,
- J. Truu,
- M. Truu,
- O. Muter,
- A. Versilovskis,
- L. Dubova, and
- Dz. Zarina
Current Research Topics in Applied Microbiology and Microbial Biotechnology01 Jan 2009
Preview Abstract
Effect of various amendments added to soils contaminated with explosives was studied under laboratory conditions. A mixture of bacterial isolates was inoculated into soils samples and incubated at +28 °C. After 14 days incubation, the pH and Eh level in the liquid fraction of soils amended with water was 6.73 and +34mV, in turn, with buffered solution was 7.2 and -30 mV, correspondingly. The total count of microorganisms in the samples was dependent on the presence of bacterial inoculum and nutrient amendments. Inoculation of soil samples with mixture of bacterial isolates had a strong effect on microbial community composition revealed by 16S rDNA-DGGE analysis. Several bacterial strains present in inoculum became dominant in TNT and RDX amended samples.

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