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The aim of this paper is to compare the biomechanical characteristics of a newly designed assembly locking compression plate (NALCP) and traditional locking compression plate (LCP) for internal fixation of femoral-shaft comminuted fractures. A femoral-shaft wedge fracture model (AO classification 32-C2.1) was created in six pairs of femoral specimens (n=12) randomly divided into two equal groups. Biomechanical properties were tested with axial and torsional loading tests. The relative maximum displacement of fracture blocks and strain was recorded. A strain diagram was made; the fatigue test results of NALCP specimens under axial load were recorded. Under axial load, the relative maximum displacement of fracture blocks in the X, Y, and Z axes was smaller in NALCP specimens than in LCP specimens (P<0.05 and 0.01, respectively). Under torsional load, the relative maximum displacement of fracture blocks in the X and Z axes in NALCP specimens was less than that in LCP specimens (P<0.01) but no statistically significant difference in the Y axes (P>0.05) was found. In both cases, the main NALCP strain was higher than the LCP strain (P<0.01) but no statistically significant difference in mean strain (P>0.05) was found. Our NALCP provides strong mechanical stability for comminuted femoral fractures and can effectively avoid stress concentration, reduce stress shielding, and facilitate bone healing.