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In this study, the surface acoustic wave device combined with an oscillator circuit was used as the excitation platform to explore the photoluminescence responses of the two-dimensional atomic layers of MoS₂. The MoS₂ layers were prepared using the mechanical exfoliation method, and then transferred onto the SAW delay line area of the platform. Under the varied servo driving voltages of the excitation platform, various power intensities of the oscillator were obtained. It represented that the various energy levels of acoustic waves were propagated underneath the MoS₂ layers. From the observation by multiphoton excitation microscopy, the excited fluorescent intensities of the MoS₂ species were detected at various levels. Studies have proved that as the driving voltage of the oscillator increases, the interaction between the MoS₂ species and the acoustic wave is also enhanced.