This paper describes the influence of surface roughness of steel plate on self-assembly behavior of silica particles based on SEM observations and the wettability of the suspension. The 304 stainless steel plate having two different surface roughness and spherical silica powder were used for the investigation. The silica layer was obtained by dipping the steel plate into the suspension and drawing it under various drawing speed. As a result, silica particle layers were formed on the plate surface when the stainless steel had a rough surface. In contrast, it was difficult to obtain the silica layers for the smooth surface.