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PIXE technique was applied to the measurement of elements in the placenta, maternal blood and cord blood of human. The elements determined in these samples include Cl, K, Ca, Fe, Cu and Zn. The values obtained by PIXE were compared with those by ICP-AES to test the accuracy of the method. In placental samples, the mean values of K, Fe, Cu and Zn concentrations obtained by the two methods agree, while Ca concentration is lower in PIXE analysis. The values from PIXE, however, show larger variations resulting from inhomogeneity of the placental samples composed of various tissues different in histological functions. In the analysis of blood samples, the results of the two methods agree for Cl, K, Ca, Fe, Cu and Zn, although the standard deviations tend to be larger in PIXE. These results indicate that PIXE is a useful method for the determination of elements in placental and blood samples, although the preparation to homogenize sample is necessary to obtain accurate results.
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With approximately 130 million babies born worldwide every year umbilical cord blood represents perhaps the largest potential source of stem cells for regenerative medicine. Between 1972 and 2008, it is estimated that over 10,000 patients would have been treated by cord blood cells for over 80 different clinical conditions. Cord blood stem cells are used clinically mostly to support patients suffering from haematological and immunological diseases but they also provide emerging therapeutic solutions for limited cases of type 1 diabetes or infant cerebral injuries. Cord blood samples are collected after birth and bio-processed before cryopreservation in either public biobanks for unrelated allogenic use or private family biobanks for related allogenic and autologous uses. Regenerative medicine research demonstrated the existence of multipotent stem cells with embryonic characteristics in cord blood, which can produce over 20 tissue types including liver, neural or insulin-secreting cells. Cord blood stem cells not only offer therapeutic benefits at present but also show real potential for the advancement of regenerative medicine.
The observations that mice exposed to otherwise lethal irradiation could survive if their spleens or marrows were shielded, or if they received an infusion of bone marrow, led to the first attemps of bone marrow transplantation in humans in the mid-1950s by E. D. Thomas and J. Ferrebee. Thanks to Thomas' persistence despite criticism and initial clinical failures, and thanks to the development of a canine model of bone marrow transplantation by Thomas and Storb, the role of allogeneic hematopoietic cell transplantation (HCT) changed during the last 50 years from a desperate therapeutic maneouver plagued by apparently insurmountable complications to a curative treatment modality for thousands of patients with hematologic diseases. Further, it was recognized that allogeneic immunocompetent cells contained in the graft mediated therapeutic antitumor effects independent of the action of the high-dose therapy. These were termed graft-versus-tumor (GVT) effects. This prompted the recent development of non-myeloablative conditioning regimens for allogeneic HCT that have allowed offering this treatment modality in elderly patients and those with comorbid conditions. While hematopoietic stem cells were identified in the early 1960s, identification of other types of stem cells such as mesenchymal stem cells or embryonic stem cells might pave the way for stem cell therapy in regenerative medicine in the future.