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Numerous additives of vegetal source are currently used for the production of “green” eco-polymers. Officinal herbs represent a precious font of polyphenols and antioxidant biomolecules with effective activity against pathogen and unwanted microorganisms. This study was focused on the use of rosemary extract and Polyvinyl alcohol (PVA) to create a polymeric mixture with antimicrobial activity against different pathogen bacteria, for packaging applications. Thus, a series of PVA-rosemary extract polymers, having different concentration of the vegetal extract, was prepared. Samples were tested against some pathogen Gram positive and Gram negative microorganisms by the halo test on agar plates. Polymeric mixture revealed a dramatic antimicrobial activity against all microorganisms tested, also when the lowest amount of the vegetal extract was used. Free extracts or PVA only were absolutely ineffective as antimicrobial agents.